HA Tag is one of the most common amino acid tags used to produce recombinant proteins. is a short 9 amino acids polypeptide sequence YPYDVPDYA derived from hemagglutinin protein, fused to recombinant proteins to aid in detection, purification, and tracking.
The XpressCheck HA Tag test strip is a competitive lateral flow assay-based technique for the rapid detection of HA tagged proteins. The assay has a simple procedure and can be completed within 3 minutes without any instrument. The strip can be applied directly to unpurified samples in cell lysis buffer or cell culture medium (with or without PBS). It is also compatible with samples in water or different buffers such as PBS, Tris, or HEPES etc.
The test strip can be used to qualitatively detect the expression of HA tagged proteins. With serial diluted samples the assay can also be carried out in a semi-quantitative manner to determine the concentration of the HA tagged protein. It is an ideal tool for protein expression evaluation, optimization and colony selection. The detection limit of the strip is about 0.2 µM, corresponding to 10 µg/mL for a protein with a molecular weight of 50 kDa.
XpressCheck is a patent pending technique with improved sensitivity, compatibility, ease of use and shelf-life compared to traditional lateral flow products.
Reviews
There are no reviews yet.