XpressQuant Mouse IgG test strip

The XpressQuant mouse IgG test strip is a novel combo lateral flow assay-based technique for the rapid detection and semi quantitative measurement of mouse IgG, and mouse IgG Fc fused proteins. The assay has a simple procedure and can be completed within 5 minutes without any instrument. The strip can be applied directly to unpurified samples in cell lysis buffer or cell culture medium (with or without FBS). It is also compatible with samples in common buffers such as PBS, Tris, or HEPES etc.

The detection limit of the XpressQuant mouse IgG test strip is 0.1 µg/mL for mouse IgG1 and IgG2a. The strip does not detect mouse IgG2b or IgG3. The quantitative range is between the detection limit to up to 10 mg/mL. Unlike traditional sandwich lateral flow assays, it is not affected by the Hook Effect and does not generate false negative results in high concentration samples. By comparing the line patten with the images provided in the protocol, sample concentrations can be semi-quantitatively determined by naked eye inspection simply.

XpressQuant is a patent pending technique. It is a first of a kind lateral flow technique that can be used to quantitatively measure analyte concentrations with unprecedented ease and speed.