XpressCheck Biotin Tag Test strip

Order through Avantor/VWR: Box of 25 / Box of 100.

Product Overview

The Instanosis XpressCheck Biotin Tag Test Strip is a high-affinity, rapid lateral flow qualitative dipstick engineered for immediate verification of protein, antibody, and nucleic acid biotinylation. Designed to eliminate the workflow bottlenecks of traditional colorimetric HABA assays or tedious streptavidin-HRP Western blotting, this protocol-ready strip confirms successful biotin conjugation from a micro-volume sample in under 3 minutes. Manufactured in the USA to rigorous quality standards, it provides a reliable, instrument-free solution for assay developers, core facilities, and biopharmaceutical researchers to evaluate labeling efficiency and batch consistency at the point of synthesis.

Technical Specifications & Key Data

This specialized lateral flow verification dipstick is optimized to deliver robust analytical feedback during bioconjugation and assay development workflows:

• Target Analyte: Biotinylated target molecules (including antibodies, recombinant proteins, peptides, and oligonucleotides).

• Assay Format: Lateral flow dipstick.

• Analytical Sensitivity Threshold: The detection limit is 0.2 µM corresponding to approximately 1 µg/mL biotinylated BSA with an average labeling ratio of 12. With serial diluted samples, the assay can also be carried out in a semi-quantitative manner to determine the biotin molar concentration.

• Micro-Volume Protocol: Requires minimal sample consumption, preserving valuable primary labeled conjugates for downstream applications.

• Rapid Development Time: Qualitative visual lines emerge and mature completely within 3 minutes.

• Product Classification: For Research Use Only (RUO). Not for use in human diagnostic procedures.

Research & Assay Development Significance

In modern biotechnology, diagnostic manufacturing, and structural biology, confirming ligand labeling before executing complex downstream protocols prevents systemic assay failures.

• Elimination of the HABA Bottleneck: Traditional 4′-hydroxyazobenzene-2-carboxylic acid (HABA) displacement assays require large sample volumes, spectrophotometers, and complex calculation steps. The XpressCheck strip provides a visual “go/no-go” binary verification line instantly.

• Assay Architecture Integrity: Validating biotinylation efficiency before assembling complex sandwich ELISAs, bead-based multiplex assays, or lateral flow platforms ensures downstream success and maximum binding capacity on streptavidin-coated solid phases.

• Batch-to-Batch Quality Control: Incorporating a rapid dipstick screening phase into standard bioconjugation processing allows core labs to easily identify compromised reagent lots, over-labeling steric hindrance, or failed NHS-ester binding chemistries.

Ordering Options & Procurement Details

• SKU: XC-BIO

• Packaging Configurations: Supplied in standard boxes of 25 or 100 individually pouched test strips (each dipstick is hermetically sealed inside a moisture-impermeable foil pouch containing an active desiccant matrix to guarantee a stable 24-month shelf life under room temperature storage conditions).

• Institutional Supply Channels: Listed and orderable through primary scientific distribution networks such as Avantor / VWR (link above Product Overview).

Frequently Asked Questions (FAQ)

• Q1: What is the primary application of the XpressCheck Biotin Tag Test Strip?

A: This rapid test strip is designed for bioconjugation quality control, allowing researchers to quickly verify whether a protein, antibody, or nucleic acid has been successfully labeled with biotin prior to running downstream applications.

• Q2: What types of biotinylated molecules can be evaluated with this dipstick?

A: The high-affinity capture matrix is compatible with a wide spectrum of biotin-tagged targets, including monoclonal and polyclonal antibodies, recombinant proteins, small peptides, and biotinylated oligonucleotides or PCR products.

• Q3: How does this test compare to a traditional spectrophotometric HABA assay?

A: While the HABA assay calculates an exact quantitative biotin-to-protein ratio using a spectrophotometer, it consumes a large amount of target material and takes significant time. The XpressCheck strip acts as a rapid, micro-volume screening alternative that provides a visual confirmation line in 3 minutes while consuming a fraction of your sample.

• Q4: Can this test strip detect over-labeling or biotinylation that blocks an antibody’s active site?

A: This strip acts as a qualitative matrix checking for the presence and accessibility of the biotin tag itself. It confirms successful conjugation to biotin, but functional target binding should be evaluated via downstream ligand-specific assays.

• Q5: What sample types can be used for this dipstick?

A: The strip can be applied directly to the samples and is compatible with matrixes such as water, PBS, Tris, HEPES etc. Please notice that most cell culture media contain free biotin and cannot be used directly.

• Q6: Is the XpressCheck Biotin Tag Test Strip intended for clinical diagnostic testing?

A: No. This product is strictly classified for Laboratory Research Use Only (RUO). It is engineered for bioconjugation optimization, reagent preparation monitoring, and assay development validation, and is not cleared for patient diagnostics.

Click to download XpressCheck Biotin Tag Test Strip instruction