Name: XpressQuant Human IgG Test Strip
SKU: XQ-hIgG
Availability: InStock

Order through Avantor/VWR: Box of 25 / Box of 100.

Product Overview

The Instanosis XpressQuant Human IgG Test Strip is a patent-pending, first-of-its-kind, rapid, high-affinity lateral flow immunoassay engineered for the immediate, semi-quantitative estimation of human Immunoglobulin G (IgG) or IgG fusion protein titers in cell culture supernatants, hybridoma harvests, and purified antibody lots. Designed to completely eliminate the workflow bottlenecks associated with multi-hour ELISAs or costly HPLC runtime schedules, this protocol-ready dipstick delivers clear visual titration lines in under 5 minutes. Manufactured in the USA to stringent quality standards, it provides an instrument-free, point-of-use screening solution for bioprocess engineers and biopharmaceutical researchers to monitor antibody expression and optimize harvest timing at the bench.

Technical Specifications & Key Data

This specialized lateral flow quantification dipstick is optimized to deliver high-efficiency analytical feedback across automated or manual antibody expression and bioprocessing workflows:

Target Analyte: Human Immunoglobulin IgG1, IgG2 and IgG4. The product does not detect human IgG3.
Assay Format: Semi-quantitative lateral flow dipstick.
Analytical Detection Range: The quantitative range is between the detection limit (0.1 µg/mL) to up to 10 mg/mL. Unlike traditional sandwich lateral flow assays, it is not affected by the Hook Effect and does not generate false negative results in high concentration samples. By comparing the line pattern with the images provided in the protocol, sample concentrations can be semi-quantitatively determined by naked eye inspection simply.
Micro-Volume Sample Consumption: Preserves valuable biopharmaceutical yields, requiring only a fraction of the sample volume consumed by traditional analytical equipment.
Rapid Development Time: Definitive semi-quantitative visual lines stabilize completely within 5 minutes.
Product Classification: For Laboratory Research Use Only (RUO). Not for use in clinical diagnostic procedures.

Research & Bioprocess Optimization Significance

In modern biopharmaceutical development, cell line engineering, and antibody manufacturing, tracking human IgG concentrations in real-time is vital for maximizing yield and protecting batch consistency.

Elimination of HPLC and ELISA Bottlenecks: Traditional Protein A HPLC chromatography and standard sandwich ELISAs require expensive instrumentation, complex calibration curves, and hours or days of turnaround time. The XpressQuant strip provides an immediate “go/no-go” yield assessment at the bioreactor site.
Hybridoma and Clone Screening: During early-stage clone selection, core facility technicians can instantly screen hundreds of culture wells to identify high-expressing human mAb clones, accelerating cell line development timelines.
Bioreactor Harvest Timing: Monitoring the daily accumulation of extracellular human IgG in cell culture media tells operators exactly when target protein accumulation peaks, avoiding unnecessary cell lysis or proteolytic degradation of the antibody batch inside the reactor.

Ordering Options & Procurement Details

SKU: XQ-hIgG
Packaging Configurations: Supplied in standard boxes of 25 or 100 individually pouched test strips (each dipstick is hermetically sealed in a heavy-duty moisture-barrier foil pouch containing an active desiccant packet to guarantee a stable 24-month shelf life at standard room temperature).
Procurement Pathways: Authorized for direct university purchasing, corporate laboratory accounts, and federal grant allocations. Instanosis products are orderable through established primary scientific distribution catalogs such as Avantor / VWR (link above Product Overview).

Frequently Asked Questions (FAQ)

Q1: What specific target does the XpressQuant Test Strip detect?

A: This rapid assay specifically targets human Immunoglobulin G (IgG1, IgG2, IgG4), making it ideal for tracking the expression of humanized or fully human monoclonal antibodies, Fc-fusion proteins, and recombinant human IgG variants in research applications.

Q2: What is the exact analytical detection range for this IgG strip?

A: The XpressQuant IgG strip is optimized for semi-quantitation between 0.1 µg/mL and 10 mg/mL. For high-titer bioreactor cultures yielding mg/mL levels, a simple pre-dilution in standard buffer allows for accurate estimation across any concentration. Unlike traditional sandwich lateral flow assays, it is not affected by the Hook Effect and does not generate false negative results in high concentration samples.

Q3: How does this test strip compare to a traditional Protein A HPLC run?

A: Protein A HPLC provides exact quantitative analysis but demands significant instrument overhead, specialized training, and processing delays. The XpressQuant strip serves as a high-velocity, instrument-free benchmarking tool that lets operators estimate IgG titers directly at the bench in minutes.

Q4: Can this strip be used with crude cell culture media or supernatants?

A: Yes. The engineered lateral flow matrix is specially formulated to tolerate complex cellular environments, allowing researchers to sample directly from crude cell culture supernatants (with or without FBS), bioreactor fluids, or raw hybridoma media without clarification steps. It is also compatible with samples in common buffers such as PBS, Tris, or HEPES etc.

Q5: Is the XpressQuant Human IgG Test Strip cleared for human diagnostic monitoring?

A: No. This product is strictly classified for Laboratory Research Use Only (RUO). It is engineered for bioprocess optimization, clone screening, and antibody engineering validation, and is not intended or cleared for monitoring serum IgG levels in patients.

Q6: Can academic and corporate research labs purchase this kit through standard contract distributors?

A: Yes. Procurement departments can secure the Instanosis Box of 25 or 100 strips using the specific product SKU through direct institutional purchase agreements or via established scientific distribution channels such as Avantor/VWR.