V5 Tag is one of the most common amino acid tags used to produce recombinant proteins. is a short 14 amino acids polypeptide sequence GKPIPNPLLGLDST derived from Simian virus 5 (SV5), now known as Human parainfluenza virus 5, fused to recombinant proteins to aid in detection, purification, and tracking.
The XpressCheck V5 Tag test strip is a competitive lateral flow assay-based technique for the rapid detection of V5 tagged proteins. The assay has a simple procedure and can be completed within 3 minutes without any instrument. The strip can be applied directly to unpurified samples in cell lysis buffer or cell culture medium (with or without PBS). It is also compatible with samples in water or different buffers such as PBS, Tris, or HEPES etc.
The test strip can be used to qualitatively detect the expression of V5 tagged proteins. With serial diluted samples the assay can also be carried out in a semi-quantitative manner to determine the concentration of the V5 tagged protein. It is an ideal tool for protein expression evaluation, optimization and colony selection. The detection limit of the strip is about 0.2 µM, corresponding to 10 µg/mL for a protein with a molecular weight of 50 kDa.
XpressCheck is a patent pending technique with improved sensitivity, compatibility, ease of use and shelf-life compared to traditional lateral flow products.
Reviews
There are no reviews yet.