XpressQuant Rabbit IgG Test Strip

Order through Avantor/VWR: Box of 25 / Box of 100.

Product Overview

The Instanosis XpressQuant Rabbit IgG Test Strip is a patent-pending, first-of-its-kind, rapid, high-affinity lateral flow immunoassay engineered for the immediate, semi-quantitative estimation of rabbit Immunoglobulin G (IgG) titers in cell culture supernatants, recombinant expression harvests, and purified antibody lots. Designed to completely eliminate the workflow bottlenecks associated with multi-hour ELISAs or costly HPLC runtime schedules, this protocol-ready dipstick delivers clear visual titration lines in under 5 minutes. Manufactured in the USA to stringent quality standards, it provides an instrument-free, point-of-use screening solution for bioprocess engineers and researchers to monitor rabbit antibody expression and optimize harvest timing at the bench.

Technical Specifications & Key Data

This specialized lateral flow quantification dipstick is optimized to deliver high-efficiency analytical feedback across automated or manual rabbit antibody expression and bioprocessing workflows:

• Target Analyte: Rabbit Immunoglobulin G (IgG) and rabbit IgG Fc-fused proteins.

• Assay Format: Semi-quantitative lateral flow dipstick.

• Analytical Detection Range: The detection limit of the XpressQuant rabbit IgG test strip is 0.1 µg/mL, and its quantitative range is between 0.1 µg/mL to above 10 mg/mL. Unlike traditional sandwich lateral flow assays, it is not affected by the Hook Effect and does not generate false negative results in high concentration samples. By comparing the line pattern with the images provided in the protocol, sample concentrations can be semi-quantitatively determined by naked eye inspection simply.

• Micro-Volume Sample Consumption: Preserves valuable biopharmaceutical yields, requiring only a fraction of the sample volume consumed by traditional analytical equipment.

• Rapid Development Time: Semi-quantitative visual lines stabilize completely within 5 minutes.

• Product Classification: For Laboratory Research Use Only (RUO). Not for use in clinical diagnostic procedures.

Research & Bioprocess Optimization Significance

In modern therapeutic antibody discovery, histopathology validation, and recombinant protein engineering, tracking rabbit IgG concentrations in real-time is vital for maximizing yield and protecting batch consistency.

• Elimination of HPLC and ELISA Bottlenecks: Traditional Protein A HPLC chromatography and standard sandwich ELISAs require expensive instrumentation, complex calibration curves, and hours or days of turnaround time. The XpressQuant strip provides an immediate yield assessment at the bioreactor or tissue culture hood.

• Accelerated Recombinant Expression & B-Cell Screening: Rabbit monoclonal antibodies are highly valued for their superior affinity and diverse epitope recognition. During transient transfection trials or single B-cell cloning workflows, technicians can instantly screen media to identify high-expressing cultures, accelerating project timelines.

• Bioreactor Harvest Timing: Monitoring the daily accumulation of extracellular rabbit IgG in cell culture media tells operators exactly when target protein accumulation peaks, avoiding unnecessary cell lysis or proteolytic degradation of the antibody batch inside the reactor.

Ordering Options & Procurement Details

• SKU: XQ-rIgG

• Packaging Configurations: Supplied in standard boxes of 25 or 100 individually pouched test strips (each dipstick is hermetically sealed in a heavy-duty moisture-barrier foil pouch containing an active desiccant packet to guarantee a stable 24-month shelf life at standard room temperature).

• Procurement Pathways: Authorized for direct university purchasing, corporate laboratory accounts, and federal grant allocations. Instanosis products are orderable through established primary scientific distribution catalogs such as Avantor / VWR (link above Product Overview).

Frequently Asked Questions (FAQ)

• Q1: What specific target does the XpressQuant Test Strip detect?

A: This rapid assay specifically targets rabbit Immunoglobulin G (IgG), making it ideal for tracking the expression of recombinant rabbit monoclonal antibodies, native rabbit polyclonal lots, and rabbit-derived fusion frameworks in research environments.

• Q2: What is the exact analytical detection range for this rabbit IgG strip?

A: The detection limit of the XpressQuant rabbit IgG test strip is 0.1 µg/mL, and its quantitative range is between 0.1 µg/mL to above 10 mg/mL. Unlike traditional sandwich lateral flow assays, it is not affected by the Hook Effect and does not generate false negative results in high concentration samples.

• Q3: How does this test strip compare to a traditional Protein A HPLC run?

A: Protein A HPLC provides exact quantitative analysis but demands significant instrument overhead, specialized training, and processing delays. The XpressQuant strip serves as a high-velocity, instrument-free benchmarking tool that lets operators estimate rabbit IgG titers directly at the bench in minutes.

• Q4: Can this strip be used with crude cell culture media or supernatants?

A: Yes. The engineered lateral flow matrix is specially formulated to tolerate complex cellular environments, allowing researchers to sample directly from crude cell culture supernatants (with or without FBS), transient transfection fluids, or raw media without clarification steps. It is also compatible with samples in common buffers such as PBS, Tris, or HEPES etc.

• Q5: Is the XpressQuant Rabbit IgG Test Strip cleared for human diagnostic monitoring?

A: No. This product is strictly classified for Laboratory Research Use Only (RUO). It is engineered for bioprocess optimization, clone screening, and antibody engineering validation, and is not intended or cleared for clinical veterinary or human diagnostic use.

• Q6: Can academic and corporate research labs purchase this kit through standard contract distributors?

A: Yes. Procurement departments can secure the Instanosis Box of 25 or 100 strips using the specific product SKU through direct institutional purchase agreements or via established scientific distribution channels such as Avantor/VWR.

Click to download XQ-rIgG Instructions