XpressQuant Mouse IgG Test Strip

Order through Avantor/VWR: Box of 25 / Box of 100.

Product Overview

The Instanosis XpressQuant Mouse IgG Test Strip (Version 2) is a patent-pending, first-of-its-kind, rapid, high-affinity lateral flow immunoassay engineered for the immediate, semi-quantitative estimation of mouse Immunoglobulin G (IgG) titers in hybridoma cell culture supernatants and purified antibody lots. Upgraded with enhanced subclass cross-reactivity and a broader dynamic range, this protocol-ready Version 2 dipstick completely eliminates the multi-hour bottlenecks of traditional ELISAs or costly HPLC schedules, delivering clear visual titration lines in under 5 minutes. Manufactured in the USA, it provides an instrument-free screening solution to accelerate clone selection and optimize bioreactor harvests directly at the bench.

Technical Specifications & Key Data

This upgraded lateral flow quantification dipstick is optimized to deliver high-efficiency analytical feedback across automated or manual mouse antibody expression and hybridoma workflows:

• Target Analyte: All mouse Immunoglobulin G (IgG) subtypes.

• Enhanced Subclass Coverage (V2): Engineered for broad, uniform affinity across all standard mouse IgG subclasses, including IgG1, IgG2a, IgG2b, IgG2c, and IgG3.

• Analytical Detection Range: Calibrated for robust visual titration across a broad dynamic window (from 0.1 µg/mL to 5 mg/mL). Unlike traditional sandwich lateral flow assays, this test is not affected by the hook effect and does not produce false-negative results in samples with high analyte concentrations. By comparing the line pattern with the reference images provided in the protocol, the sample concentration can be semi-quantitatively determined by simple visual inspection with the naked eye.

• Micro-Volume Sample Consumption: Consumes minimal sample volume, preserving valuable primary monoclonal antibody yields for downstream functional validation.

• Rapid Development Time: Semi-quantitative visual lines stabilize completely within 5 minutes.

• Product Classification: For Laboratory Research Use Only (RUO). Not for use in clinical diagnostic procedures.

Research & Hybridoma Optimization Significance

In modern monoclonal antibody development, functional screening, and hybridoma cell line engineering, tracking mouse IgG concentrations in real-time is vital for maximizing yield and protecting batch consistency.

• Elimination of HPLC and ELISA Bottlenecks: Traditional Protein A or G HPLC chromatography and standard sandwich ELISAs require expensive instrumentation, complex calibration curves, and hours or days of turnaround time. The XpressQuant Version 2 strip provides an immediate yield assessment at the bench.

• Accelerated Hybridoma Clone Screening: During early-stage clone selection, core facility technicians can instantly screen hundreds of culture wells to identify high-expressing mouse mAb clones, accelerating cell line development timelines.

• Bioreactor Harvest Timing: Monitoring the daily accumulation of extracellular mouse IgG in cell culture media tells operators exactly when target antibody accumulation peaks, avoiding unnecessary cell lysis or proteolytic degradation of the mAb batch inside the reactor.

Ordering Options & Procurement Details

• SKU: XQ-mIgG

• Packaging Configurations: Supplied in standard boxes of 25 or 100 individually pouched test strips (each dipstick is hermetically sealed in a heavy-duty moisture-barrier foil pouch containing an active desiccant packet to guarantee a stable 24-month shelf life at standard room temperature).

• Procurement Pathways: Authorized for direct university purchasing, corporate laboratory accounts, and federal grant allocations. Instanosis products are orderable through established primary scientific distribution catalogs such as Avantor / VWR (link above Product Overview).

Frequently Asked Questions (FAQ)

• Q1: What specific target does the XpressQuant Version 2 Test Strip detect?

A: This rapid assay specifically targets mouse Immunoglobulin G (IgG), making it ideal for tracking the expression of mouse monoclonal antibodies, mouse IgG-fused proteins, hybridoma secretion levels, and recombinant mouse IgG variants in research applications.

• Q2: Does the Version 2 strip recognize all mouse IgG subclasses?

A: Yes. The upgraded Version 2 architecture features an advanced anti-mouse antibody pair optimized to recognize and resolve mouse IgG1, IgG2a, IgG2b, IgG2c and IgG3 subclasses with uniform binding affinity.

• Q3: How does this test strip compare to a traditional Protein A HPLC run?

A: Protein A HPLC provides exact quantitative analysis but demands significant instrument overhead, specialized training, and processing delays. The XpressQuant Version 2 strip serves as a high-velocity, instrument-free benchmarking tool that lets operators estimate mouse IgG titers directly at the bench in minutes.

• Q4: Can this strip be used with crude cell culture media or supernatants?

A: Yes. The engineered lateral flow matrix is specially formulated to tolerate complex cellular environments, allowing researchers to sample directly from crude cell culture supernatants (with or without FBS), bioreactor fluids, or raw hybridoma media without clarification steps. It is also compatible with samples in common buffers such as PBS, Tris, or HEPES etc.

• Q5: Is the XpressQuant Mouse IgG Test Strip cleared for human diagnostic monitoring?

A: No. This product is strictly classified for Laboratory Research Use Only (RUO). It is engineered for hybridoma screening, clone selection, and antibody engineering validation, and is not intended or cleared for clinical veterinary or human diagnostic use.

• Q6: Can academic and corporate research labs purchase this kit through standard contract distributors?

A: Yes. Procurement departments can secure the Instanosis Box of 25 or 100 strips using the specific product SKU through direct institutional purchase agreements or via established scientific distribution channels such as Avantor/VWR.

Click to download XQ-mIgG Instructions.