The XpressQuant rabbit IgG test strip is a novel combo lateral flow assay-based technique for the rapid detection and semi quantitative measurement of both rabbit IgG, and rabbit IgG Fc fused proteins. The assay has a simple procedure and can be completed within 5 minutes without any instrument. The strip can be applied directly to unpurified samples in cell lysis buffer or cell culture medium (with or without FBS). It is also compatible with samples in common buffers such as PBS, Tris, or HEPES etc.
The detection limit of the XpressQuant rabbit IgG test strip is 0.1 µg/mL, and its quantitative range is between 0.1 µg/mL to 10 mg/mL. Unlike traditional sandwich lateral flow assays, it is not affected by the Hook Effect and does not generate false negative results in high concentration samples. By comparing the line patten with the images provided in the protocol, sample concentrations can be semi-quantitatively determined by naked eye inspection simply.
XpressQuant is a patent pending technique. It is a first of a kind lateral flow technique that can be used to quantitatively measure analyte concentrations with unprecedented ease and speed.
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